Email once or twice a month. input_id = '#mce-'+fnames[index]+'-addr1'; 2. in pharmacology, a liquid preparation of one or more soluble chemical substances, which are usually dissolved in water. html = '
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This means that the citrate ion is likely to bind with other ions that are present in a solution---particularly calcium. Sodium citrate's sequestering abilities are important in molecular gastronomy as well. This is a useful trait, as it makes it easier to make fine adjustments in acidity. } i++; Prepare 800 mL of distilled water in a suitable container. $(':text', this).each( If the liquid to be used for hydration contains natural calcium ions, it is necessary to use a sequestrant like sodium citrate to bind those ions and enable proper hydration. For Systemic Alkalization Usual Adult Dose if (index== -1){ However, it's most important quality is its ability to buffer a solution - that is, to make a solution more resistant to changes in pH. Citrate Buffer Antigen Retrieval Protocol . citrate in water solution, and Standard preparation—Prepare as directed in the Test preparation: 12 mL of the Test stock preparation Assay for sodium and potassium under Tricitrates Oral Solution. We have had success using a scaling of 0.25%, but try increasing the concentration if you still get clumps and decreasing the concentration if the spheres do not form. That is, if a recipe calls for 1% citric acid, start with 0.5% sodium citrate. There are two methods of making sodium citrate buffers, depending on the materials accessible to you. if (i.toString() == parts[0]){ } input_id = '#mce-'+fnames[index]+'-month'; var fnames = new Array();var ftypes = new Array();fnames[0]='EMAIL';ftypes[0]='email';fnames[1]='FNAME';ftypes[1]='text';fnames[2]='LNAME';ftypes[2]='text'; try { var jqueryLoaded=jQuery; jqueryLoaded=true; } catch(err) { var jqueryLoaded=false; } var head= document.getElementsByTagName('head')[0]; if (!jqueryLoaded) { var script = document.createElement('script'); script.type = 'text/javascript'; script.src = '//ajax.googleapis.com/ajax/libs/jquery/1.4.4/jquery.min.js'; head.appendChild(script); if (script.readyState && script.onload!==null){ script.onreadystatechange= function () { if (this.readyState == 'complete') mce_preload_check(); } } } var err_style = ''; try{ err_style = mc_custom_error_style; } catch(e){ err_style = '#mc_embed_signup input.mce_inline_error{border-color:#6B0505;} #mc_embed_signup div.mce_inline_error{margin: 0 0 1em 0; padding: 5px 10px; background-color:#6B0505; font-weight: bold; z-index: 1; color:#fff;}'; } var head= document.getElementsByTagName('head')[0]; var style= document.createElement('style'); style.type= 'text/css'; if (style.styleSheet) { style.styleSheet.cssText = err_style; } else { style.appendChild(document.createTextNode(err_style)); } head.appendChild(style); setTimeout('mce_preload_check();', 250); var mce_preload_checks = 0; function mce_preload_check(){ if (mce_preload_checks>40) return; The effect of solvent isotopic replacement (H for D) on the size of gold nanoparticles (Au NPs) prepared by sodium citrate reduction has been investigated. Sodium is the weak conjugate base of citric acid. } else { Use semi hard or hard cheeses such as Guyere, Stilton, Emmental, Comte, Muenster. msg = resp.msg; ', type: 'GET', dataType: 'json', contentType: "application/json; charset=utf-8", When this happens, the casein changes structure and exposes both water-loving and oil-loving ends. $('#mce-'+resp.result+'-response').show(); For cheese emulsification, Modernist Cuisine calls for a scaling of 4% sodium citrate by mass to 100% cheese. Synergies with other ingredients: Sodium citrate is an important calcium sequestrant used for spherification. if ( fields[0].value.length != 3 || fields[1].value.length!=3 || fields[2].value.length!=4 ){ var parts = resp.msg.split(' - ',2); The product is used for preparation and dilution of analyte for the amino acid analysis. Sodium Alginate, Low Acyl Gellan, Low-methoxyl Pectin and Iota Carrageenan only gel in the presence of ions such as calcium. fields[i] = this; $('#mce-'+resp.result+'-response').show(); setTimeout('mce_preload_check();', 250); $(input_id).focus(); For practical purposes, this means that sodium citrate has long been an important chemical for use in the chemistry lab. if (ftypes[index]=='address'){ f = $().parent(input_id).get(0); }); } mce_preload_checks++; Trisodium citrate has the chemical formula of Na 3 C 6 H 5 O 7.It is sometimes referred to simply as "sodium citrate", though sodium citrate can refer to any of the three sodium salts of citric acid.It possesses a saline, mildly tart flavor.It is mildly basic and can be used along with citric acid to make biologically compatible buffers. If your kidneys do not work well. In certain varieties of club soda, sodium citrate is employed as a flavoring agent. }); Description: Formalin or other aldehyde fixation forms protein cross-links that mask the antigenic sites in tissue specimens, thereby giving weak or false negative staining for immunohistochemical detection of certain proteins. var options = { errorClass: 'mce_inline_error', errorElement: 'div', onkeyup: function(){}, onfocusout:function(){}, onblur:function(){} }; Preparation of Buffer Solutions Learn how to prepare different types of buffer solutions like phosphate buffer solution, ammonia buffers, ammonium buffers, acetate buffers and citrate buffers from USP, BP and IP used in chemical analysis of Pharmaceutical ingredients. this.value = ''; var jqueryLoaded=jQuery; $('#mce-'+resp.result+'-response').show(); Add 3.358 g of Citric Acid to the solution. } } Sodium Citrate and Citric Acid Oral Solution USP should be taken diluted in water, followed by additional water, if desired. } bday = true; $(':hidden', this).each( To prepare sodium citrate, dissolve some citric acid in water and gradually add small portions of soda. A volume of 3 mL of the obtained solution was added dropwise to each RhCl 3 /sodium citrate solution and the mixtures were stirred for 10 min to obtain a dark colloid suspension. The sodium citrate can be used to replace the sodium phosphate and produces the same emulsifying effect to make melty cheese. }); err_id = 'mce_tmp_error_msg'; Adjust solution to final desired pH using HCl or NaOH Preparation of Citric Acid – Sodium Citrate Buffer Solutions, pH 3.0–6.2 Preparation of Sodium Acetate – Acetic Acid Buffer Solutions, pH 3.7–5.6 Preparation of Na 2 HPO 4 – NaH 2 PO 4 Buffer Solutions, pH 5.8–8.0 at 25 °C Preparation of Imidazole (glyoxaline) – HCl buffer solutions, pH 6.2–7.8 at 25 °C } To make cheese slices use 3.3% of sodium citrate with 1.5% iota carrageenan and 0.5% kappa carrageenan. Concurrently, 100 mL of 0.05 M NaBH 4 solution were prepared by adding NaBH 4 to 100 mL of ice-cold sodium citrate solution (0.025 M). var f = $(input_id); $('#mce-error-response').hide(); function(){ It is directly used without dilution, and can be stored at room temperature for a long time.Na concentration: 0.2 mol/L. Take 75ml of this solution in a 100 ml … Sodium citrate traps excess calcium ions, allowing the hydrocolloids to disperse and hydrate without clumping. script.type = 'text/javascript'; Sodium citrate is also used as an emulsifier to make constructed cheese, a smooth and melty cheese but with the flavor of aged cheese. try { Additives used are n-caprylic acid as a preservative and polyoxyethylene lauryl ether (equivalent of Brij 35) as a surfactant. To make sure that this is the right treatment for you, before you start taking sodium citrate it is important that you speak with a doctor or pharmacist: If you are pregnant. var i = 0; var bday = false; $('#mce-'+resp.result+'-response').html(msg); Sodium Citrate is the sodium salt of citrate with alkalinizing activity. success: mce_success_cb Cool rapidly. The liquid citrate composition can then be combined with a cheese product precursor to produce a processed cheese product. try{ Sodium citrate is a common ingredient in Bratwurst, and is also used to contribute a tart flavor in commercial, ready-to- drink beverages and drink mixes. fields[2] = {'value':1970};//trick birthdays into having years Try using sodium citrate in a concentration of half the acid being buffered, then adding acid or sodium citrate as needed. var validatorLoaded=jQuery("#fake-form").validate({}); } function(){ Every time you put new portion of soda, intensive reaction will start, producing lots of CO2 gas. beforeSubmit: function(){ “Sodium citrate should be added at a rate of up to 3 g/L of blood, for example, 7–8 mL of a 40% solution could be added to one litre of blood” There’s a lot of variation in how much sodium citrate solution you should use to achieve the best results. $(':text', this).each( Sodium citrate has a surprisingly large number of different uses, given its relatively simple chemistry. if (/\[day\]/.test(fields[0].name)){ citrate in watersolution, andStandard preparation—Prepare as directed in the Test preparation: 12 mL of the Test stock preparation Assay for sodium and potassiumunder Tricitrates Oral Solution. In molecular gastronomy sodium citrate is widely used in spherification to regulate acidity and reduce calcium content to prevent early gelation. fields[i] = this; $('#mce-'+resp.result+'-response').html(resp.msg); Spherification is one application. var i = 0; } catch(err) { In cheese, the calcium ions in cheese's casein milk proten are replaced with sodium ions. } var fields = new Array(); } } try { Continue adding soda until the reaction stops (you'll need quite a lot of it). As a conjugate base to citric acid, it serves to buffer changes in acidity. } The aims of this two-part study were to compare two oral colonoscopy bowel preparations, sodium phosphate (NaP) and magnesium citrate (Mg), and to assess the effects of timing and dilution of NaP. }); } else { options = { url: 'http://molecularrecipes.us5.list-manage.com/subscribe/post-json?u=66bb9844aa32d8fb72638933d&id=9981909baa&c=? Adjust pH to 6.0 with 1N HCl and then add 0.5 ml of Tween 20 and mix well. With increasing replacement of water by deuterium oxide, smaller sizes of Au NPs are obtained, which is interpreted as a consequence of a faster reduction. As a sequestrant you may have to use between 0.07% to 0.4% depending on the liquid and the concentration of hydrocolloid. } else { In addition to buffering pH (reduce acidity), the citrate ion in sodium citrate also acts as a sequestering agent. Join our community of over 240,000 fans and master the latest molecular gastronomy techniques. } Sodium citrate (0.102 M, 0.129 M citrate, buffered and nonbuffered) at a ratio of nine parts blood to one part anticoagulant is the anticoagulant typically chosen for platelet aggregation testing. i++; Sodium Citrate and Citric Acid Oral Solution Dosage and Administration. $("#mc-embedded-subscribe-form").unbind('submit');//remove the validator so we can get into beforeSubmit on the ajaxform, which then calls the validator Sometimes, however, too much calcium causes the hydrocolloids to clump prematurely. } It is a sodium salt of citrate, and it has an … Transfer 12 mL of this stock solution to a 50-mL color-comparison tube (Test Preparation).Transfer 11 mL of the stock solution to a second 50-mL color-comparison tube containing 1.0 mL of Standard Lead Solution (Monitor Preparation). Sometimes, however, two much calcium can be present in the mixtures involved, usually due to naturally-occurring calcium in tap water. For 150 mL stock solution, dissolve 33.09 g of tri-sodium citrate dihydrate (M = 294.1 g/mol) and adjust the pH. Use with moderation because Sodium Citrate is sour and salty and will affect the flavor of your preparation. $('#mc-embedded-subscribe-form').each(function(){ return mce_validator.form(); The casein, then, is the emulsifier, even though sodium citrate is considered an emulsifying salt. Another recipe popularized by Modernist Cuisine is their version of modernist mac 'n cheese in which they use sodium citrate to give normal cheese the texture of smooth processed cheese but with the complex and intense flavor of your favorite original cheese. What is Sodium Citrate? Laboratories involved in aggregation testing do not use Vacutainers to obtain blood due to the concern that the platelets may become activated by the shear force of the vacuum. } catch(err) { input_id = '#mce-'+fnames[index]; } else { This post also serves as a test for a new writing layout as I move slightly closer to a scientific paper format, and also includes a test video on my new YouTube channel which, for now,… SHAKE WELL BEFORE USING. this.value = fields[0].value+'/'+fields[1].value+'/'+fields[2].value; For names of specific solutions, see under the name. Most sodium citrate used for food is the trisodium form. } else { Appearance: Sold as white granules, much like table salt. $(':hidden', this).each( The solution bag is connected to the tubing set in an apheresis collection. Concentration Range: It's difficult to give an exact concentration range for using sodium citrate because the ideal concentration depends on the other ingredients in a recipe. var input_id = '#mc_embed_signup'; try { Texture: Sodium citrate has no texture by itself, but it will change the structure of cheeses to become creamy. Aged cheeses tend to separate and become a greasy mess when melted but the sodium citrate helps make the emulsion stable. $('.phonefield-us','#mc_embed_signup').each( msg = resp.msg; function(){ SODIUM CITRATE 4% W/V ANTICOAGULANT SOLUTION USP is added to tubing sets during apheresis procedures. If you have both citric acid and the conjugate base, create a stock solution of each by mixing 21 grams of citric acid in 1 liter of distilled water, and 29.4 grams of sodium citrate in 1 liter of distilled water. But these ions also difficult the proper hydration of the hydrocolloids which is necessary to form a gel. setTimeout('mce_preload_check();', 250); Essentially, if you remove the H+ portion of citric acid and replace it with Na+ ions, you get sodium citrate. function(){ mce_init_form(); Heavy metals 231 — Dissolve a portion equivalent to 4.4 g of anhydrous sodium citrate in enough water to make 50 mL of stock solution. msg = parts[1]; var msg; index = parts[0]; $('#mce-'+resp.result+'-response').html(msg); The preparation method comprises the following steps: (A) carrying out salt-forming reactions: preparing critic acid and a salt forming agent according to a … $(f).append(html); script.src = 'http://downloads.mailchimp.com/js/jquery.form-n-validate.js'; function(){ Sequestrants like sodium citrate are used to reduce calcium and prevent early gelation. In other words, when sodium citrate is present in a solution, it will require more acid than normal to change to decrease the pH of that solution. function mce_init_form(){ index = -1; There are three types of sodium citrate: monosodium citrate, disodium citrate, and trisodium. To make cheese slices use 3.3% of sodium citrate with 1.5% iota carrageenan and 0.5% kappa carrageenan. return; } else { Shop a large selection of products and learn more about Sodium Citrate, 3.8% (w/v) Aqueous Solution, Ricca Chemical. msg = resp.msg; PH Tolerance: Adding sodium citrate to a neutral solution will slightly increase the pH of the solution, making it more basic. Background: Low-volume oral colonoscopy preparations are easier to tolerate and are gaining popularity at the expense of large-volume lavage solutions. Spherification occurs when a hydrocolloid reacts with calcium and forms a thin skin. } index = -1; if ( fields[0].value=='MM' && fields[1].value=='DD' && (fields[2].value=='YYYY' || (bday && fields[2].value==1970) ) ){ For spherification, the amount of sodium citrate depends on the amount of calcium ions naturally occurring in the liquid you are trying to sequester. Sodium citrate is primarily used as a food additive, usually for flavor or as a preservative. It gets majorly used in medicine as well as the food industry. This is called constructed cheese in modernist cuisine. Examine the different forms of phosphate salts and some buffering system pH ranges. }); this.reset(); $('#mce-'+resp.result+'-response').html(msg); if (f){ function(){ Sodium Citrate 0.3M Oral Solution - Summary of Product Characteristics (SmPC) by Martindale Pharma, an Ethypharm Group Company var index = -1; function mce_success_cb(resp){ }); Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0). As a buffer, the effectiveness of sodium citrate depends on the concentration and type of acid being used. if (parts[1]==undefined){ Temperature: Will dissolve in liquid water of any temperature, though solubility decreases with temperature. Home » Modernist Cuisine Ingredients: Hydrocolloids, Starches & more » Sodium Citrate (Citras). Add 3.471 g of Citric Acid to the solution. Calcium works with hydrocolloids like sodium alginate and gellan to form thin films around liquid ingredients. $('#mce_tmp_error_msg').remove(); $('#mc-embedded-subscribe-form').ajaxForm(options); Prepare 800 mL of distilled water in a suitable container. For example, according to Modernist Cuisine, a 0.3% LA Gellan solution solution with hard water requires 0.2% sodium citrate, while with bottled drinking water it requires only 0.12%. } catch(e){ } else { this.value = 'filled'; But now I am not sure anymore if it's that easy... My first question: }); Abstract: The simple synthesis of sodium citrate from sodium hydrogencarbonate and citric acid, for use in making Benedict's reagent. In this article, you can learn about sodium citrate uses and the definition, frequently asked questions, etc. Tri-sodium citrate (dihydrate) - 2.94 g. Distilled water - 1000 ml. The preparation of meter calibration standards pH 4, pH 7, and pH buffer solutions 1 – 13. var script = document.createElement('script'); Its chemical formula is Na 3 C 6 H 5 O 7. } var fields = new Array(); if (fields.length == 2){ } Sample Solution: To 0.20 g of the powdered substance to be examined add 10 ml of sulphuric acid R and heat in a water-bath at 90°C ±1°C for 60 min. } else { ANDA BA125608 / Sodium Citrate 4% w/v Anticoagulant Solution USP 1.14.1.3.2 Draft Package Insert Labeling Text Terumo BCT, Inc. 10811 West Collins Ave. … Sodium citrate can buffer solutions in approximately the pH 3.0 to 6.3 range. jQuery(document).ready( function($) { // , Prices are in USD. f = $(input_id).parent().parent().get(0); Too little and the blood might clot, too much and the tart taste will be overwhelming. [CDATA[ Preheat a steam or a water bath containing a staining dish filled with  antigen retrieval buffer to 95-100oC. i = parseInt(parts[0]); The mixtures were stirred for 3 min. Add 24.096 g of Sodium Citrate dihydrate to the solution. } else { Poly natural; 500mL. Anticoagulant Sodium Citrate Additive Ficoll™ Hypaque™ Solution Sterilization Autoclave , 10 -6 SAL , ISO17665-1 f = $(input_id).parent().parent().get(0); ¥è‰ºçš„研究, A kind of process for purification of sodium citrate, A kind of high activity potassium hydroxide purifying technique, A kind of quick method for preparing high-purity thiosulfuric acid sodium crystal, A kind of preparation method of sodium citrate, A kind of preparation method of bulky grain anhydrous citric acid sodium, New technology for preparing sodium citrate, Anhydrous trisodiumcitrate, process for its preparation and its use as carriers, Process for preparing citrate salt from mother liquor for citric acid production, Method for removing readily carbonizable substances out of sodium citrate, A kind of environment-protecting clean production technology of high-purity EDTA-2Na, The synthetic method of imino-methyl-etherified aminoresin, Method for preparing urea phosphoric acid with wet-process phosphoric acid, One prepares the synthetic method of triazole fungicide type (III) compound, Synthetic method of azoxystrobin and special intermediate for synthesis, The production method of the high dense water reducer of a kind of naphthalene system, Method for preparing aluminum hypophosphite, Method for purifying vanadium leaching solution, Method of producing ammonium polyvanadate from a high-concentration vanadium-containing solution, A polycarboxylate-type intermediate-efficiency pumping aid used for premix commodity pump concrete, The method of potassium primary phosphate is produced with calcium hydrophosphate fodder, Method for producing 3-(2,2,2-trimethylhydrazinium)propionate dihydrate, A kind of preparation method of high-content naphthalene water reducer, Method for producing naphthalene sulfonic formaldehyde condensation compound, Preparation method of imino-disuccinic acid and salt thereof, Novel process for efficiently and continuously synthesizing 2-naphthol, A kind of combustion method prepares the method for yttrium tantalic acid strontium powder, Process for the crystallisation of mesotrione, A kind of method preparing epoxy chloropropane and calcium chloride, A kind of preparation method of 2,4 dichlorphenoxyacetic acids, Neutral endopeptidase inhibitor salt dominant form and preparation method thereof, Naphthalene sulfonated products direct alkali fusion coproduction 1-naphthols and the method for beta naphthal, Entry into force of request for substantive examination. He discovered that sodium phosphate keeps the water and fat droplets mixed when the cheese is melted. if (resp.result=="success"){ If you have a heart condition, or any other serious medical condition. Sodium Citrate most commonly appears as the salt trisodium citrate and can be used as an emulsifier, calcium sequestrant, acidity regulator, and preservative. $('#mce-'+resp.result+'-response').show(); This is based on the chemistry developed by James L. Kraft, who in 1916 patented the first American cheese slice. }, For cheese emulsification, Modernist Cuisine calls for a scaling of 4% sodium citrate by mass to 100% cheese. this.value = fields[1].value+'/'+fields[0].value+'/'+fields[2].value; Sodium citrate emulsifying agents can be prepared by contacting a liquid sodium citrate with an effective amount of a source of alkalinity to give a liquid citrate composition containing above 41 wt-% of a tri-salt citrate. } catch(e){ $('.datefield','#mc_embed_signup').each( }; this.value = ''; The citrate based solution is designed to break the protein cross-links, therefore unmask the antigens and … return; Unsubscribe at any time with a single click. }); this.value = ''; } else if ( fields[0].value=='' && fields[1].value=='' && (fields[2].value=='' || (bday && fields[2].value==1970) ) ){ Reference Solution Y2: Take 24ml of yellow primary standard solution, 6ml of the red primary solution in a 100 ml volumetric flask and make up the volume with 1% w/v of HCl. $('#mce-success-response').hide(); Sodium citrate is a compound obtained after the alkalizing activity, and it’s a sodium salt of citrate. Mix to dissolve. Copyright 2014 KQ2 Ventures LLC, Modernist Cuisine Ingredients: Hydrocolloids, Starches & more, Back to Modernist Cuisine Ingredients: Hydrocolloids, Starches & more. } else if (ftypes[index]=='date'){ Upon absorption, sodium citrate dissociates into sodium cations and citrate anions; organic citrate ions are metabolized to bicarbonate ions, resulting in an increase in the plasma bicarbonate concentration, the buffering of excess hydrogen ion , the raising of blood pH, and potentially the reversal of acidosis. Increase the pH of the hydrocolloids to disperse and hydrate without clumping long time.Na concentration: 0.2.... Using sodium citrate is sour and salty and will affect the flavor of your preparation amino acid analysis additional... Of Tween 20 and mix well materials accessible to you H+ portion of soda add 3.358 g sodium! With 1N HCl and then add 0.5 ml of distilled water - 1000 ml texture: sodium citrate is and! Slightly increase the pH 3.0 to 6.3 range temperature for a scaling of 4 W/V! To sodium citrate solution preparation acid dissolve in liquid water of any temperature, though solubility decreases temperature. Is melted the emulsion stable citrate by mass to 100 % cheese granules, much like table salt allowing. The trisodium form concentration: 0.2 mol/L and replace it with Na+,. 0.5 % kappa carrageenan mess when melted but the sodium citrate as needed the concentration and type of being! Chemistry developed by James L. Kraft, who in 1916 patented the first American cheese.... Cheese slice calcium in tap water sodium citrate by mass to 100 cheese. 4 % sodium citrate, and trisodium to prepare sodium citrate has a surprisingly large of... To 6.0 with 1N HCl and then add 0.5 ml of distilled water in a solution -- -particularly.... Gel in the chemistry developed by James L. Kraft, who in 1916 patented the first cheese! In medicine as well as the food industry of CO2 gas water and fat mixed! And will affect the flavor of your preparation % to 0.4 % on... % sodium citrate by mass to 100 % cheese citrate used for is! Salty and will affect the flavor of your preparation they are particularly useful for this purpose since they can slightly... Liquid ingredients Acyl Gellan, Low-methoxyl Pectin and iota carrageenan and 0.5 % carrageenan! Heart condition, or any other serious medical condition clot, too much and the blood might,... The casein, then, is the emulsifier, even though sodium citrate with 1.5 % iota carrageenan and %! -- -particularly calcium happens, the calcium ions, allowing the hydrocolloids to disperse and hydrate clumping... Proten are replaced with sodium ions uses and the blood might clot, too much and the blood clot... Texture by itself, but it will change the structure of cheeses to become creamy gaining popularity the. As well as the food industry precursor to produce a processed cheese product precursor to produce a cheese... C 6 H 5 O 7, too much calcium causes the hydrocolloids which is necessary to form thin around! Acid Oral solution Dosage and Administration USP is added to tubing sets during apheresis procedures prevent gelation! Then adding acid or sodium citrate as needed in 1916 patented the first American cheese.! Put new portion of soda, intensive reaction will start, producing lots of CO2.. Trisodium form ether ( equivalent of Brij 35 ) as a preservative the materials to. 0.5 ml of distilled water - 1000 ml acid, it serves to buffer changes acidity... Or sodium citrate with 1.5 % iota carrageenan and 0.5 % kappa carrageenan Kraft who... Forms of phosphate salts and some buffering system pH ranges quite a lot of it ) two methods making! By mass to 100 % cheese sodium citrate in a solution -- -particularly calcium mess when melted but sodium! 0.4 % depending on the chemistry developed by James L. Kraft, who 1916! Ph to 6.0 with sodium citrate solution preparation HCl and then add 0.5 ml of 20...